Intact Genomics (IG™) Recombineering Chemically Competent Cells enable high-efficiency transformation for DNA, plasmid, and BAC engineering, and homologous recombination. Derived from SW102 (a 10B background strain), they feature a defective lambda prophage and a galK deletion for efficient DNA/BAC modification via galK positive/negative selection. The cells are tetracycline resistant (5 μg/mL).
- High transformation efficiency.
- Supports DNA, plasmid, and BAC engineering, plus homologous recombination.
- Enables precise modification using galK positive/negative selection.
- Derived from SW102 (10B background).
- Heat-inducible prophage with red genes (exo, bet, gam), repressed at 32 °C and induced at 42 °C.
- Tetracycline resistant (TetR: 5 μg/ml).